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The CSPCA and the Charitable Trust have joined forces to benefit the future health of our breed with the formation of the DNA Bank.
The Charitable Trust has agreed to pay the $10.00 per sample charged by the
University of Missouri for the first 250 samples received.
Two members of the Trust's Board will join two members of the CSPCA DNA Databank Committee and the President of the CSPCA to round out the Sample Designee Committee. This Committee will oversee the future use of the samples.
DNA BANK – FORMS AND HOW TO:
The DNA Bank is designed to store DNA samples for current and future research and testing, as DNA tests become available.
The DNA is isolated from blood, tissue, or frozen semen samples submitted by owners and stored by Dr. Gary Johnson at the, University of Missouri College of Veterinary Medicine. Banked samples can be withdrawn by either Dr. Johnson or the Chinese Shar-Pei Club of America for approved research projects to benefit the health of our breed.
The CSPCA DNA Bank Committee urges all breeders/owners of Chinese Shar-Pei to send in samples from their dogs as the first step in solving the problem of amyloidosis in our breed.
Plans for a blood draw to be held at Gap Nationals are being finalized but for those of you who aren’t planning on attending Gap Nationals the Health Survey, blood sample protocol, litter forms and vet letter are available here for downloading.
The cost of extracting the DNA will be $10 per sample to be
paid to the University of Missouri by the Charitable Trust. The only cost to the
owner(s) is having the blood drawn (and several local clubs have organized blood
draws where this is done for either no fee or a minimal fee) and shipping the
blood overnight to the University of Missouri (again at all the organized blood
draws the cost of shipping the blood has been borne by the organizing club).
For a more detailed explanation of this project please read the article that will be printed in the September/October Barker.
CSPCA DNA Bank Committee
Grace Fritz
Jerry Doka
Kim Albrecht
Susie Lauer
SAMPLE HANDLING
For Canine DNA Research at the University of Missouri
Blood Sample - The ideal sample for DNA extraction is 5-10cc’s of whole blood, in purple-topped (EDTA) tubes. For very small dogs or puppies, 3ccs should be sufficient. The blood sample needs only to be put in the tubes and rocked gently a few times to distribute the anticoagulant - do not spin, extract serum, or anything further. Refrigerate if the sample is being held for any time before shipping.
Frozen Semen - If there is frozen semen stored from sires or affected dogs, DNA can be extracted from it. Please send 2 straws. They do not need to be shipped frozen, but do pack them in a crush-proof container.
Tissue Sample - Tissue removed as a result of surgery, or an organ sample upon death of the dog will provide a large amount of DNA for research. Please discuss this with your vet ahead of time if you intend to do this. (If the dog is to be euthanized, have a blood sample pulled first, if possible, and send both samples.) First choice is spleen, second choice kidney, and third choice is liver (a piece about the size of your thumb is all that is needed - not the entire organ). One tissue sample is sufficient. Have the organ removed as soon as possible following death, place into a labeled freezer bag, put that into a second bag, freeze, and ship.
Label sample with the following;
call name - owner’s last name
(If samples from several dogs are sent together, number samples and forms)
An Individual Dog Information form should be completed, and a pedigree copy must be included with the sample to tie it in with the correct family. If the dog is not affected but is a relative of an affected, please indicate the relationship.
Shipping - Ideally the sample should be shipped immediately (with a tissue sample make certain it is completely frozen first). If samples are held for a day or over a weekend, blood must be refrigerated, and tissue samples must be kept frozen. Ship via overnight delivery (US Mail, UPS, or FedEx). Do not send on a Friday - there will not be anyone to accept the delivery on a weekend, and the sample could be unusable by Monday. Pack in a small insulated container (most vets have these for shipping samples to labs), with one or more cool packs - it is important that blood samples be kept cool but not frozen, and tissue samples be kept as frozen as possible.
The delivery address is;
Dr. Gary Johnson - (Breed of Dog) DNA Research
320 Connaway Hall
University of Missouri
Columbia, MO 65211
If you need clarification, or have any questions about any of these procedures, please contact Liz Hansen by phone (573-884-3712), email (HansenL@missouri.edu), or regular mail (321 Connaway Hall, University of Missouri, Columbia, MO 65211). Liz is Dr. Johnson’s Project & Information Coordinator, and can help with any questions you may have.
Thank you for your cooperation and participation!